Fig. 3

miR-155enhanced expansion of virus-specific CD8⁺ T cells. WT and miR-155 ^−/− mice were i.p. infected with DM-JHMV. Spleens were removed at day 8 p.i., and virus-specific T cells were identified by tetramer staining. Representative dot blots indicated that while similar frequencies and numbers of virus-specific CD4⁺ T cells (a) were present in WT and miR-155 ^−/− mice, there was a significant (**p < 0.001) decrease in both the frequency and numbers of virus-specific CD8⁺ T cells (b) in miR-155 ^−/− mice compared to WT mice. Histograms are presented as average ± SEM; statistical significance was measured using unpaired, one-tailed Student’s T tests. Data presented are derived from two independent experiments with a minimum of four mice/experimental group. ^* p < 0.05; ^** p < 0.01; ^*** p < 0.001