Fig. 5

Obesity and Aβ increase hippocampal inflammation. a Representative images of IBA-1 (red) and GFAP (green) immunoreactivity in the CA1 and dentate gyrus regions of the hippocampus. b Obesity and Aβ infusion, either alone or in combination, increased IBA-1 immunoreactivity (IBA-1-IR) in the CA1 and dentate gyrus regions of obese and Aβ-infused mice. c Obesity combined with Aβ infusion synergistically increased GFAP immunoreactivity (GFAP-IR) in the CA1 and dentate gyrus regions of the hippocampus. d Association between hippocampal ¹¹C-PBR-28 accumulation at 3 months and GFAP immunoreactivity. e Association between hippocampal ¹¹C-PBR-28 accumulation at 3 months and IBA-1 immunoreactivity. f Representative hippocampal cytokine antibody microarray blots from control + vehicle, control + β, obese + vehicle, and obese + Aβ hippocampal samples. Inflammatory markers identified as significant predictors of ¹¹C-PBR-28 signals are circled in green. Inflammatory markers significantly correlating with ¹¹C-PBR-28 signals are circled in red. Data presented as mean ± SEM. p < 0.015