Fig. 3

Teriflunomide influences GM-CSF-mediated microglial proliferation in vitro. Microglia were treated without (control) or with GM-CSF (5 ng/ml) in mixed glial cell cultures for 48 h. Cultures were incubated with 10 μM BrdU for the last 16 h, and dividing microglia were then visualized by labeling with a FITC-conjugated anti-BrdU antibody. For comparison of untreated (0 μM) and teriflunomide-treated (0.25–5 μM) samples (n = 4), a paired t test was used followed by correction for multiple comparisons by Benjamini and Hochberg. P values <0.05 were considered significant. Asterisks denote a significant difference versus the indicated control (*p < 0.05)