Fig. 6

IFNγ blocks pericyte migration independently of PDGF-BB. a, b Cell migration was measured by scratching wells after 48 h of IFNγ (1 ng/mL) treatment and measuring the number of cells that moved into the gap area after a further 48 h with vehicle or PDGF-BB (10 ng/mL) treatment. Representative images of Coomassie-stained pericytes are presented in a, and manual counts of cells in the gap area (normalized to vehicle-treated condition), plotted as mean ± s.e.m. (n = 3) ^####, ****(p < 0.0001), **(p < 0.01), (two-way ANOVA), are presented in b Scale, 500 μm. c Magnified view of pericytes from migrating edge of scratch wound from conditions in a Scale bar, 100 μm