Journal of Neuroinflammation

Table 1 Primary antibodies and primers used in this study

From: Andrographolide induces Nrf2 and heme oxygenase 1 in astrocytes by activating p38 MAPK and ERK

Target	Antibody	Format	Dilution^a	Source Company
OH-1	OH-1	Rabbit polyclonal	1:1000; 1:200 (IF)	Abcam
Nrf2	C-20	Rabbit polyclonal	1:1000	Santa Cruz
pS40-Nrf2	phos-Nrf2	Rabbit monoclonal	1:1000	Abcam
Nqo1	C-19	Goat polyclonal	1:1000	Santa Cruz
Keap1	E-20	Goat polyclonal	1:1000	Santa Cruz
Total Erk1/2	Erk1/2	Rabbit polyclonal	1:1000	Cell Signaling
pT202/pY204-Erk	phos-Erk	Rabbit polyclonal	1:1000	Cell Signaling
Total p38	p38	Rabbit polyclonal	1:1000	Cell Signaling
pT180/pY182-p38	D3F9	Rabbit monoclonal	1:1000	Cell Signaling
β-actin	AC-15	Mouse monoclonal	1:5000	Sigma-Aldrich
Lamin B1	Lamin B1	Rabbit polyclonal	1:10,000	Abcam
TBP	TBP	Mouse monoclonal	1:5000	Abcam
Ubiquitin	P4D1	Mouse monoclonal	30 μL per 1 mg lysate protein (IP)^b	Santa Cruz
Target	Forward primer		Reverse primer
OH-1	5′-GGCTCTCTTTTCTTGGGCCT-3′		5′-GCCTCTACCGACCACAGTTC-3′
Nrf2	5′-CAGTCTTCACCACCCCTGAT-3′		5′-CAGTGAGGGGATCGATGAGT-3′
Nqo1	5′-GCGAGCGGGGAAAATACTCT-3′		5′-CCTCCTGCCCTAAACCACAG-3′
β-actin	5′-ACCCGCGAGTACAACCTTCT-3′		5′-TTCTGACCCATACCCACCAT-3′
GAPDH	5′-CTCATGACCACAGTCCATGC-3′		5′-TTCTGACCCATACCCACCAT-3′

^aStated dilutions are for immunoblotting except for immunofluorescence (IF) staining and immunoprecipitation (IP). Source companies are Abcam (Cambridge, UK); Cell Signaling Technology (Danvers, MA, USA); Santa Cruz Biotechnology (Dallas, TX, USA); and Sigma-Aldrich (St. Louis, MO, USA)
^bConjugated with agarose beads (500 μg antibody/0.25 mL agarose)

Back to article page

ISSN: 1742-2094

Contact us

Submission enquiries: journalsubmissions@springernature.com