Fig. 7

Quantification of CD45⁺ cells in injured nerves following perineural transfer of CFSE-labeled polarized macrophages. a Representative dot blots of CD45 staining: (left panel) CD45 FMO-negative control; (middle panel) CD45⁺ cells after one injection of DMEM only; (right panel) CD45⁺ cells following one injection of M2 macrophages. b Total number of CD45⁺ cells per nerve after one injection of DMEM only or CFSE-labeled M0, M1, and M2 cells. c Total number of CD45⁺ cells per nerve after two injections of DMEM only or CFSE-labeled M0, M1, and M2 cells. In all experiments, DMEM- or CFSE-labeled macrophages were injected at the injury site on day 14 (one injection) or on days 14 and 15 (two injections) after CCI. Animals were killed 24 h after the first injection and 24 h after the second injection, and cells from the nerves were analyzed using flow cytometry and FlowJo software. *p < 0.05, **p < 0.01, ***p < 0.001 (one-way ANOVA, Bonferroni’s test); ^## p < 0.01; ^### p < 0.001 compared to same treatment 24 h after the first injection (unpaired t test). Data show the mean ± SEM (n = 6 animals per group)