Fig. 2
From: Acute neuroinflammation provokes intracellular acidification in mouse hippocampus
Neuroinflammation induced by i.p. injections of LPS provoked intracellular acidification in the CA1 neurons. a Representative examples of ratiometric pHi measurements in the BCECF-AM-loaded slices from 19-month-old mice. The images were taken with the excitation wavelengths 440 and 490 nm, and the F 440/F 490 ratio was assessed. Regions of interest (ROI) used for evaluation of the pHi levels are shown by circles. Bar = 100 μm. b Quantification of the pHi levels. Mean ± SEM. n = 41 (3-month saline), 60 (3-month LPS), 10 (19-month saline), and 12 (19-month LPS); *p = 0.04, ***p = 0.0001