Fig. 1

IFNAR restricts TBEV replication in astrocytes. Primary astrocytes were isolated from WT and IFNAR^−/− mice and infected with Hypr MOI 0.1. a Cell were stained 72 hpi with GFAP (astrocytes), TBEV E protein, and DAPI. Scale bar 193 μm. Primary astrocytes (b, c) and MEF (d, e) were infected with TBEV (MOI 0.1), and viral growth was determined over time by focus forming assay (b, d) and qPCR (c, e). Data are cumulative from at least two independent experiments performed in triplicates (n = 6). Asterisks indicate data were statistically significant: ***p < 0.0001