Fig. 5

Flow cytometric analysis of M1 and M2 macrophages in control and Cstb ^−/− spleen and brain. a Illustrative plots show the flow cytometric gating strategy of nucleated spleen cells and enriched brain mononuclear cells. (i) In the spleen, CD45⁺F4/80⁺ macrophages were divided into CD45⁺F4/80⁺MHCII⁺CD206⁻ M1 and CD45⁺F4/80⁺MHCII^−/+CD206⁺ M2 cells. (ii) In the brain, the CD45^hiF4/80⁺ macrophage population was divided into CD45^hiF4/80⁺MHCII⁺CD206⁻ M1 and CD45^hiF4/80⁺MHCII^−/+CD206⁺ M2 macrophages. Ratio between M1 and M2 macrophages at P14 and P30 (M1:M2 ratio) in the b spleen (n = 6 samples) and c brain (n = 15 samples per genotype at P14 and n = 11 per genotype at P30). Data are presented as mean ± SEM (*p < 0.05, ***p < 0.001)