Fig. 1

Tollip is highly expressed in dopaminergic neurons. a Representative Tollip (in green) and DAPI staining (in blue) in 10-month-old female Tollip WT mice. Up - position of the analyzed sections in the Paxinos Atlas [84]. Inset shows the substantia nigra pars compacta (SNc), the hippocampus (hipp), and the cortex (ctx) analyzed below. Magnification = ×63. Scale bar = 10 μm. Bar graphs below represent the number of Tollip-positive cells relative to DAPI-positive cells. (n = 3 WT mice); **P < 0.01; ***P < 0.001; one-way ANOVA, Holm–Sidak’s post hoc test. b Representative Tollip (in green) and tyrosine hydroxylase (TH) (in red) immunostainings and DAPI staining (in blue) in the SNc of 9-month-old male Tollip WT mice. Magnification = ×20. Scale bar = 50 μm. White insets show the region photographed on the right, at high magnification (×63); scale bar = 50 μm. Bar graph indicates that 98.3 ± 0.3% of TH-positive cells display also Tollip immunostaining and 88.3 ± 6.3% of Tollip-positive cells are TH immunopositive