Fig. 3

The impact of dietary substitution of saturated by unsaturated fats on the activity of the RvD2 system in the hypothalamus. Six-week-old Swiss mice were included in the study and fed on chow or HF for 16 weeks. Another group of mice was assigned to HF for 8 weeks following 8 weeks on a HF supplemented with 30% omega 3 (HFS) (a). Relative quantification of the RvD2 was made in the hypothalamus of mice fed either chow (CT), the high-fat diet (HF) or the HF supplemented with 30% omega 3 (HFS) (b). The transcript expressions of tumour necrosis factor alpha (TNFα) (c) and interleukin-1 beta (IL1β) (d) were evaluated using real-time PCR in samples collected from the hypothalamus of mice. Caloric intake (e) and body mass (f) were measured during the experimental period. At the end of the treatment, the experimental groups were subjected to a glucose tolerance test and an insulin tolerance test, and results are shown as the area under the curve (AUC) (g) and constant of glucose decay (kITT) (h). In all experiments, n = 7. In b–h, *p < 0.05 vs. CT and #p < 0.05 vs. HF