Fig. 5

Involvement of EP2 in the expression of genes related to classical microglial activation. mRNA expression of iNOS (a, b), NOX2 (c, d), G6PD (e, f), and VEGFA (g, h) in microglia. a, c, e, g LPS increases mRNA expression at 24 h, and an EP2 antagonist (1 μM PF 04418948) reduces the effect of LPS (three to four independent experiments). b, d, f, h An EP2 agonist (1 μM butaprost) increases the mRNA expression of these genes at 8 h (three independent experiments). *p < 0.05, **p < 0.01, ***p < 0.001 vs. control; &p < 0.05 vs. LPS; ^# p = 0.06 vs. LPS. “EP2 antag” indicates EP2 antagonist