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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Enhanced neuroinflammation mediated by DNA methylation of the glucocorticoid receptor triggers cognitive dysfunction after sevoflurane anesthesia in adult rats subjected to maternal separation during the neonatal period

Fig. 6

TSA decreased the percentage of methylated clones within the exon 17 GR promoter region and increased the expression of GR in the hippocampal tissues in adult MS rats. A volume of 2 μl of TSA (100 ng/ml in DMSO) was infused into the lateral ventricle of MS rats for seven consecutive days. a The frequency of methylation observed at each CpG site in the exon 17 GR promoter region showed that TSA decreased the percentage of methylated clones relative to the DMSO treatment. b qRT-PCR (GAPDH as internal control) showed that TSA increased the mRNA levels of GR in MS rats compared with the DMSO treatment. c TSA treatment increased the nuclear GR protein levels in the hippocampus of MS rats relative to the DMSO treatment. * P < 0.05, ** P < 0.01 versus DMSO. Error bars represent the means ± SD (n = 6). Statistical analyses were performed with Student’s two-sample t test

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Journal of Neuroinflammation

ISSN: 1742-2094