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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: WWL70 attenuates PGE2 production derived from 2-arachidonoylglycerol in microglia by ABHD6-independent mechanism

Fig. 8

Determination of WWL70 inhibitory effect on PGE2 and PGE2-G productions using LC/MS/MS. BV2 cells treated with LPS (0.1 μg/ml) for 8 h were replaced with fresh non-serum medium containing 2-AG with/without WWL70 and incubated for 30 min. PGs in the medium was extracted with SPE, then employed LC-MS/MS together with internal standards PGE2-d4 and PGE2-G-d5 to determine the quantity of PGE2 (a) and PGE2-G (b). nd denotes not detected. Data are represented as mean ± SD (n = 3 or 4)

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