Fig. 5

Mice lacking xCT in immune cells are less sensitive to EAE disease than mice expressing xCT in immune cells. Clinical scores of BM-transplanted mice expressing (n = 13) or lacking (n = 14) xCT in their immune cells are different 11 and 14 days after EAE induction (a), as well as highest score reached (c). No differences were observed in AUC (b). On day 15, the spinal cord was collected, sliced, and stained for demyelination (d, e), infiltration (f), and microglial activation (g) and subsequently scored (n = 4–5). Scoring was performed blinded. Data are expressed as mean ± SEM, Mann-Whitney U test, *p < 0.05. Representative images of LFB (d), MBP (e), cresyl violet (f), and Iba1 (g) staining of the lumbar section of EAE-induced mice transplanted with xCT^+/+ or xCT^−/− BM are shown below each graph (scale bar, 100 μm)