Fig. 1
From: Rab32 connects ER stress to mitochondrial defects in multiple sclerosis
Proteomic characterization of MS brain tissues. a Normal-appearing white matter (NAWM) in the right frontal lobe exhibiting intact LFB staining for myelin. b Identification of lesion and NAWM areas in autopsy tissue sections of an MS patient brain derived from a 44-year-old male patient. Hematoxylin and eosin staining in a chronic lesion in the pons shows a hypocellular center (upper left) containing neuronal cells with diminished LFB staining for myelin and hypercellular edge. c Abundant CD68-immunoreactive macrophages/microglia are detected in the hypercellular edge of the active lesion. d Western blotting analysis showing the amounts of Rab32, together with ER stress-related markers (calnexin, BiP/GRP78, GRP94, and CHOP (patient 1: secondary-progressive MS, patient 2: relapsing-remitting MS)). e Western blotting analysis showing the amounts of MAM-related proteins GRP75, Drp1, mitofusin2, and PACS-2. f Expression of Rab32 associated with selected marker proteins in EAE (GRP75 and CHOP) in tissues obtained from the spinal cords of EAE mice. Triplicate samples from CFA naive, EAE disease peak (clinical grade 1), and EAE post-peak (clinical grade 4) are shown