Fig. 5

Assessment of Rab32-induced mitochondrial dynamics and neurite length in HFNs. a, b Primary human neurons were transfected with pDsRed2-Mito alone (Control: a) or co-transfected with pDsRed2-Mito and Rab32Q85L (b). Twenty-four hours post transfection, cells were processed for immunofluorescence microscopy. Mitochondria and FLAG distribution are shown as indicated. Scale bar 5 μm. c Quantification of the number of mitochondria within neurites, expressed as number of mitochondria per micrometer. d Quantification of the average neurite length/cell. For panels c and d, n = 30, *p < 0.05, and **p < 0.01, EGFP was used as an alternative control. e Immunoelectron microscopic determination of mitochondria phenotype. SH-SY5Y cells were transfected with FLAG-tagged Rab32Q85L, followed by immunogold detection of the FLAG signal. Mitochondria from expressing and control (non-expressing) cells were measured, and their number of cristae was determined (table below, units: μm). Scale bar 0.5 μm