Fig. 1

Minocycline and everolimus reduced nitrite production under both LPS and kainic acid treatment, while rapamycin only reduced nitrite production under kainic acid treatment in BV2 cell line. After treatment with different drugs for 24 hours, nitrite was assayed. Both LPS (n = 10) and kainic acid (KA, n = 4) increased nitrite production significantly. Minocycline (LM and KM groups, n = 5 and 4, respectively) and everolimus (LE and KE groups, n = 10 and 5, respectively) attenuated the increased nitrite levels under both LPS and KA treatment,. Rapamycin, however, attenuated the elevated nitrite level only under KA treatment (KR group, n = 5), and it had no effect on the nitrite level under LPS treatment (LR group, n = 5). ***p < 0.001; *p < 0.05, compared with the control group (Ctl, n = 17). ^### p < 0.001; ^## p < 0.01; ^# p < 0.05, compared with the LPS and KA groups, respectively. Data are presented as mean ± SEM. Ctl control, Eve/E everolimus, KA/K kainic acid, LPS/L lipopolysaccharide, Min/M minocycline, Rap/R rapamycin