Fig. 4
From: Activation of human B cells negatively regulates TGF-β1 production
Stimulation of B cells elicits secretion of IL-6, TNF, and IL-10, but not TGF-β1. Purified B cells from human blood were cultured with serum-free medium alone or with combined CpG-B + anti-Ig for 24 h. The amount of (a) IL-6, (b) TNF, (c) IL-10, and (d) total TGF-β1 protein in the culture cell-free supernatants was determined by ELISA. Bar graphs show mean cytokine concentrations (± SEM) from technical triplicates from one representative donor out of two analyzed