Fig. 2

Analysis of Obr and pSTAT3 expression in the hypothalamus. a Confocal images showing Obr (green) in neurons immunostained with anti-NeuN (red) staining in EMD and control mice. Scalebar = 20 μm. b Percent of Obr⁺ cells that were NeuN⁺ diminished in EMD mice compared to control mice. c Confocal images showing pSTAT3 (green), and NeuN (red) staining in EMD and control mice. Scalebar = 20 μm. d Quantitation of pSTAT3-positive NeuN neurons in hypothalamic sections of EMD and control mice. Data are presented as mean ± SEM. ARC arcuate nucleus. *P < 0.05, **P < 0.01; Student’s t test.