Fig. 2

TLR4 is expressed in cultured rat hippocampal neurons and expression levels increases with in vitro aging. a Representative immunofluorescence images for TLR4 of young (8 DIV) and aged (20 DIV) hippocampal neurons. Control (Ctrl) refers to images taken from similar cultures in the absence of primary antibody. Scale bar is 10 μm and applies to all frames. b Quantitative analysis of immunofluorescence intensity levels (Optical Density in arbitrary units) for TLR4. Bars represent mean ± SEM (n = 40–115 cells from 3–4 independent cultures). *p < 0.05 aged vs young. c Cells were fixed and tested for immunofluorescence detection of TLR4 in neurons. Pictures correspond to a bright field image, immunostaining of β III tubulin and TLR4 in the same neurons. Nuclei were stained using DAPI. Data are representative of three independent experiments