Fig. 5

Effects of MK801 on Ca²⁺ responses to LPS and NMDA in aged cultures of rat hippocampal neurons. Hippocampal neurons cultured for >18 DIV were loaded with fura2 and subjected to Ca²⁺ imaging. Traces show representative single-cell [Ca²⁺]_cyt responses to 1 μg/ml LPS and NMDA 100 μM in the absence (a) and the presence of 10 μM (b) or 50 μM (c) MK801. Bars represent average rises in [Ca²⁺]_cyt calculated as in Fig. 3 induced by NMDA (d) or LPS (e) in the absence (control) and the presence of MK801. Data are from 4–8 independent experiments. Data are from 39 (control), 15 (MK801 10 μM), and 14 (MK801 50 μM) neurons studied in 8, 4, and 5 independent experiments, respectively. *p < 0.05 vs control