Fig. 4

Signaling deficits not associated with a decline in the rate of transport. a Displacement vs time of retrograde vesicles in BDNF or BDNF + IL-1β conditions. Endosomes traveled at an average of 0.35 (n = 10 cultures used to acquire the data) and 0.5 (n = 8) μm/s. Therefore, IL-1β does not reduce the rate of transport of BDNF-GFP vesicles. Graph is mean ± range of all values. Transport data were collected from axons running through a single microfluidic groove (<10). b Ratio of endosomal velocity to speed, indicating directedness of motion. There is a very minimal difference between the means. Mean ± SE; BDNF = 0.93 ± 0.3, n = 10 vs BDNF + IL-1β = 0.87 ± 0.5, n = 8. IL-1β does not alter the character of vesicle locomotion. n refers to the number of cultures used to acquire the data. c 3D kymographs of BDNF and d BDNF + IL-1β treatment groups. The 3D kymograph shows the position/time motion of a single vesicle from each group (X-axis = position, Y-axis = time, Z-axis = intensity)