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Table 2 Antibodies used in experiments

From: Microglia-derived IL-1β contributes to axon development disorders and synaptic deficit through p38-MAPK signal pathway in septic neonatal rats

Antibody Host Company Cat.No. Application Concentration
Phos-p38 Rabbit Cell Sigaling Technology, Danvers, MA, USA 4511S Cell (WB) 1:1000
p38 Rabbit Cell Sigaling Technology, Danvers, MA, USA 8690S Cell (WB) 1:1000
β-actin Mouse Cell Sigaling Technology, Danvers, MA, USA 3700S Tissue/cell (WB) 1:1000
IL-1β Rabbit Chemicon International, Temecula, CA, USA AB1832P Tissue (WB/IF) 1:1000
IL-1R1 Rabbit Santa Cruz Biotechnology, Santa Cruz, CA, USA SC689 Tissue (WB/IF) 1:100
NFH Mouse Sigma-Aldrich, Saint Louis, MO, USA N0142 Tissue/cell (WB) 1:1000
NFM Mouse Sigma-Aldrich, Saint Louis, MO, USA N2787 Tissue/cell (WB/IF) 1:1000
NFL Mouse Sigma-Aldrich, Saint Louis, MO, USA N5139 Tissue/cell (WB/IF) 1:1000
MAP-2 Mouse Abcam, Cambridge, MA, USA ab11267 Cell (IF) 1:500
Synaptophysin Mouse Abcam, Cambridge, MA, USA ab8049 Tissue (IF) 1:200
Synaptophysin Rabbit Abcam, Cambridge, MA, USA ab14692 Tissue/cell (WB) 1:200
PSD-95 Rabbit Abcam, Cambridge, MA, USA ab18258 Tissue (WB/IF) 1:500
PLP Rabbit Abcam, Cambridge, MA, USA ab28486 Tissue (WB/IF) 1:500
Alexa Fluor555 Goat Invitrogen Life Technologies Corporation A-21422 Tissue/cell (IF) 1:200
Alexa Fluor555 donkey Invitrogen Life Technologies Corporation A-31572 Tissue/cell (IF) 1:200
Alexa Fluor488 donkey Invitrogen Life Technologies Corporation A-21202 Tissue/cell (IF) 1:200
Lectin   Sigma-Aldrich, St. Louis, MO L2886 Tissue/cell (IF) 1:100