Microglia-derived IL-1β contributes to axon development disorders and synaptic deficit through p38-MAPK signal pathway in septic neonatal rats

Table 2 Antibodies used in experiments

Antibody	Host	Company	Cat.No.	Application	Concentration
Phos-p38	Rabbit	Cell Sigaling Technology, Danvers, MA, USA	4511S	Cell (WB)	1:1000
p38	Rabbit	Cell Sigaling Technology, Danvers, MA, USA	8690S	Cell (WB)	1:1000
β-actin	Mouse	Cell Sigaling Technology, Danvers, MA, USA	3700S	Tissue/cell (WB)	1:1000
IL-1β	Rabbit	Chemicon International, Temecula, CA, USA	AB1832P	Tissue (WB/IF)	1:1000
IL-1R₁	Rabbit	Santa Cruz Biotechnology, Santa Cruz, CA, USA	SC689	Tissue (WB/IF)	1:100
NFH	Mouse	Sigma-Aldrich, Saint Louis, MO, USA	N0142	Tissue/cell (WB)	1:1000
NFM	Mouse	Sigma-Aldrich, Saint Louis, MO, USA	N2787	Tissue/cell (WB/IF)	1:1000
NFL	Mouse	Sigma-Aldrich, Saint Louis, MO, USA	N5139	Tissue/cell (WB/IF)	1:1000
MAP-2	Mouse	Abcam, Cambridge, MA, USA	ab11267	Cell (IF)	1:500
Synaptophysin	Mouse	Abcam, Cambridge, MA, USA	ab8049	Tissue (IF)	1:200
Synaptophysin	Rabbit	Abcam, Cambridge, MA, USA	ab14692	Tissue/cell (WB)	1:200
PSD-95	Rabbit	Abcam, Cambridge, MA, USA	ab18258	Tissue (WB/IF)	1:500
PLP	Rabbit	Abcam, Cambridge, MA, USA	ab28486	Tissue (WB/IF)	1:500
Alexa Fluor555	Goat	Invitrogen Life Technologies Corporation	A-21422	Tissue/cell (IF)	1:200
Alexa Fluor555	donkey	Invitrogen Life Technologies Corporation	A-31572	Tissue/cell (IF)	1:200
Alexa Fluor488	donkey	Invitrogen Life Technologies Corporation	A-21202	Tissue/cell (IF)	1:200
Lectin		Sigma-Aldrich, St. Louis, MO	L2886	Tissue/cell (IF)	1:100

ISSN: 1742-2094