Fig. 2
Sodium butyrate increases microglial/macrophage cell number in the ipsilateral hemisphere after neonatal hypoxia-ischemia. Seven-day-old rats (PND7) were subjected to hypoxia-ischemia followed by 6 days of recovery. SB or vehicle was administered directly after the onset of HI and for 5 consecutive days. Brain sections were stained for ED1 immunoreactivity (red). a Confocal photomicrographs show immunohistochemical reaction in the frontal cortex of ipsilateral (injured) and contralateral (control) hemispheres with or without SB treatment. Numerous ED1-positive cells are mainly seen in ipsilateral side, and their number further increases after SB administration. Lower panel represents magnification of the upper ipsilateral photomicrographs. Scale bar 100 μm. b Graph shows the number of ED1-labeled cells quantified in the frontal cortex (1.44 mm2 area). The values represent means ± SD of five animals per each experimental group. The one-way ANOVA and Bonferroni test indicate significant differences in the number of ED1(+) cells between the investigated groups: ***p < 0.001. IPSI ipsilateral hemisphere, CONTRA contralateral hemisphere