Fig. 2

Neonatal BCG vaccination enhanced the effect of Enr on hippocampal neurogenesis but does not affect neuroglia differentiation. a–d Representative micrographs of the DG stained for BrdU (red) and with DCX staining (a, green), BrdU/NeuN (b), BrdU/Iba-1 (c), and BrdU/GFAP (d) from the PBS-Reg, BCG-Reg, PBS-Enr, and BCG-Enr mice. e–h Quantification of BrdU⁺/DCX⁺ cells (e), BrdU⁺/NeuN⁺ cells (f), BrdU⁺/Iba-1⁺ cells (g), and BrdU⁺/GFAP⁺ cells in the DG among all of the groups. *p < 0.05, two-way ANOVA, followed by LSD post hoc test; n = 6 per group. Scale bars 50 μm. The data are presented as the means ± SEMs