Fig. 2

Factor XII (FXII) deficiency and pharmacologic inhibition of activated FXII displays blood-brain barrier stabilizing and antiedematous effects 1 and 3 days after induction of focal trauma. a Fluorometric measurement of Evans Blue extravasation into the brain parenchyma after 2 h, 12 h, 1 day (d1), and 3 days (d3) in mice treated with rHA-Infestin-4 or vehicle (n = 5–6 per group, *P < 0.05, unpaired, two-tailed Student’s t test). b Fluorometric measurement of Evans Blue extravasation into the brain parenchyma after 1 (d1) and 3 days (d3) in wildtype (WT) mice and FXII-deficient (FXII^−/−) mice (n = 5–6 per group, ***P < 0.001, *P < 0.05, unpaired, two-tailed Student’s t test). Right panel shows representative brain slices of Evans Blue extravasation into the tissue. c Antiedematous effect in FXII deficiency and FXIIa inhibition shown by determination of brain water content in the lesioned hemisphere 24 h after trauma (n = 8–9, *P < 0.05, unpaired, two-tailed Student’s t test)