Fig. 2

Longitudinal imaging of nestin induction/expression following transient MCAO in nestin-luc-GFP mice reveals a long-term induction of neurogenesis. a–f In vivo imaging of nestin signal induction after MCAO shows the activation of nestin promoter up to 2 weeks post-ischemia. The color calibrations at right are photon counts. Representative images showed a collection of imaging samples at six different wavelengths (560–660 nm) across the emission spectrum of the bioluminescent source (firefly luciferase) with the substantial fraction of light over 600 nm. g Plot of the data obtained by measuring the luciferase activity at the site of ischemic lesion (in photon per second, p/s). The solid black line shows the nestin induction after MCAO. A strong induction of the promoter at 24 h after MCAO (MCAO vs baseline **p = 0.0154, n = 5) with a peak of expression at 72 h, (MCAO vs baseline *p = 0.0347, n = 5) as well as smaller peak of expression at 7 days was noted. h 3D reconstruction of bioluminescent signal at 24 h after MCAO in nestin-luc-GFP mice. Using DLIT algorithms and structural images of the mouse, these data were transformed into 3D images. The more intense signal is seen in the frontal part of the brain of the mouse and strong activation of signal on the site of ischemia