Fig. 5

Increased number of GFAP- and nestin-positive cells after acute and chronic LPS administration. Immunofluorescence analysis of nestin and GFAP expression 1 day after LPS showed an increased number of positive cells in the SVZ (a, b, d, e) and DG (g, h, j, k). 14 days of LPS administration lead to a decrease in the number of NPGs in both neurogenic regions (c, f, i, l). All presented panels came from independent staining experiments. Immunofluorescence analysis of GFAP and Ki67 reveals that a small number of Ki67+ cells are also GFAP positives in the SVZ (m–o) and DG (p–r) 1 day after LPS. Statistical analysis of nestin- (s) and GFAP- (t) positive cells have been performed in both the SVZ and DG regions. As by immunofluorescence experiment, a significant increase of nestin and GFAP expression was observed after 24 h after LPS. The white arrowheads (o–t) show the double positive cells. Statistical analysis was performed by one-way ANOVA followed by Tukey’s multiple test *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (n = 5). Scale bar: 100 μm