Fig. 7

Proliferative response of nestin⁺/Iba-1⁺ cells after inflammatory stimuli. a–l A strong proliferative response, seen by the immunoreactivity for Ki67, is induced in the brain 72 h after MCAO in the SA (a–d), DG (e–h), and SVZ (i–l) regions. The vast majority of the proliferative cells are CD11b⁺, and very few nestin⁺/CD11b⁺/Ki67⁺ can be found in the 3 regions studied (white arrowheads). 24 h after LPS injection, Ki67 immunoreactivity is found in the DG (m–p) and SVZ (q–t). The Ki67⁺ cells are either CD11b⁺ or nestin⁺, and very few cells in proliferation express both markers (white arrowheads). Primary glial cell culture showed a strong induction of nestin immunoreactivity and proliferation after LPS treatment in CD11b⁺ cells (white arrowheads) (u–bb). Scale bar: 50 and 100 μm