Fig. 7

Lipopolysaccharide-stimulated MP increase neuroinflammation in the cortex of uninjured mice. Enriched MP were isolated from control and LPS-stimulated BV2 microglia and were treated ± PEG-TB (6 μl/100 μl) prior to being stereotactically injected into the cortex of adult male C57BL/6 mice. Markers of cortical neuroinflammation were measured at 24 h postinjection. There was a significant increase in pro-inflammatory mediators (IL-1β, TNF-α, miR-155, IL-6, and NOS2) in the cortex of LPS MP-injected mice (**p < 0.01, ***p < 0.001 vs control MP-injected group). Neutralization of LPS MP prior to injection resulted in a significant decrease in each pro-inflammatory mediator (^p < 0.05, ^^p < 0.01, ^^^p < 0.001 vs LPS MP-injected group; one-way ANOVA with Student-Newman-Keuls correction for multiple comparisons; n = 6/group). Bars represent mean ± S.E.M.