Suppression of MyD88-dependent signaling alleviates neuropathic pain induced by peripheral nerve injury in the rat

Table 1 List of primary and secondary antibodies used for Western blot analysis

Antibody	Host	Company	Catalog number	Dilution	incubation conditions
MyD88 ^a	rabbit	Abcam	ab2064	1:500	Overnight 4 °C
TRIF ^b	rabbit	Abcam	ab13810	1:1000	Overnight 4 °C
IL-1β	rabbit	Proteintech	16806-1-AP	1:500	Overnight 4 °C
IBA1	rabbit	Proteintech	10904-1-AP	1:500	Overnight 4 °C
TNF-α	rabbit	Sino Biological	80045-RP02	1:500	Overnight 4 °C
GFAP	rabbit	Cell signaling technology	#12389	1:1000	Overnight 4 °C
HMGB1	rabbit	Cell signaling technology	#6893	1:1500	Overnight 4 °C
ERK	rabbit	Cell signaling technology	#4695	1:2000	Overnight 4 °C
pERK	rabbit	Cell signaling technology	#4370	1:1000	Overnight 4 °C
NF-κB	rabbit	Cell signaling technology	#8242	1:1000	Overnight 4 °C
pNF-κB	mouse	Cell signaling technology	#13346	1:1000	Overnight 4 °C
β-actin	mouse	Santa Cruz Biotechnology	TA-09	1:1000	Overnight 4 °C
Anti-rabbit IgG horseradish peroxidase (HRP)	Goat	ZSGB-BIO	ZDR-5306	1:3000	1 h RT
Anti-mouse IgG horseradish peroxidase (HRP)	Goat	ZSGB-BIO	ZDR-5307	1:3000	1 h RT

^a MyD88 antibody (Abcam, catalog #ab2064) was used for IHC or WB in 18 research articles (PubMed ID: 27389279, 26191134, 25446227, 26224622, 24307174, 24410883, 24477912, 24719558, 25187650, 23479602, 21278343, 20212458, 20837465, 20456021, 19553541, 17503341, 17114422, 16517734, 16461741)
^b TRIF antibody (Abcam, catalog #ab13810) was used for IHC or WB in 11 research articles (PubMedID: 27389279, 26191134, 24146036, 22361049, 20511556, 19265160, 19648648, 19389833, 17503341, 17314314, 17000866)

ISSN: 1742-2094