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Table 1 List of primary and secondary antibodies used for Western blot analysis

From: Suppression of MyD88-dependent signaling alleviates neuropathic pain induced by peripheral nerve injury in the rat

Antibody Host Company Catalog number Dilution incubation conditions
MyD88 a rabbit Abcam ab2064 1:500 Overnight 4 °C
TRIF b rabbit Abcam ab13810 1:1000 Overnight 4 °C
IL-1β rabbit Proteintech 16806-1-AP 1:500 Overnight 4 °C
IBA1 rabbit Proteintech 10904-1-AP 1:500 Overnight 4 °C
TNF-α rabbit Sino Biological 80045-RP02 1:500 Overnight 4 °C
GFAP rabbit Cell signaling technology #12389 1:1000 Overnight 4 °C
HMGB1 rabbit Cell signaling technology #6893 1:1500 Overnight 4 °C
ERK rabbit Cell signaling technology #4695 1:2000 Overnight 4 °C
pERK rabbit Cell signaling technology #4370 1:1000 Overnight 4 °C
NF-κB rabbit Cell signaling technology #8242 1:1000 Overnight 4 °C
pNF-κB mouse Cell signaling technology #13346 1:1000 Overnight 4 °C
β-actin mouse Santa Cruz Biotechnology TA-09 1:1000 Overnight 4 °C
Anti-rabbit IgG horseradish peroxidase (HRP) Goat ZSGB-BIO ZDR-5306 1:3000 1 h RT
Anti-mouse IgG horseradish peroxidase (HRP) Goat ZSGB-BIO ZDR-5307 1:3000 1 h RT
  1. a MyD88 antibody (Abcam, catalog #ab2064) was used for IHC or WB in 18 research articles (PubMed ID: 27389279, 26191134, 25446227, 26224622, 24307174, 24410883, 24477912, 24719558, 25187650, 23479602, 21278343, 20212458, 20837465, 20456021, 19553541, 17503341, 17114422, 16517734, 16461741)
  2. b TRIF antibody (Abcam, catalog #ab13810) was used for IHC or WB in 11 research articles (PubMedID: 27389279, 26191134, 24146036, 22361049, 20511556, 19265160, 19648648, 19389833, 17503341, 17314314, 17000866)