Fig. 1

ZIKV infectivity of primary human retinal endothelial cells. Phase contrast images of a an uninfected confluent monolayer of retinal endothelial cells, b a confluent monolayer of retinal endothelial cells staining positive for von Willebrand factor (VWF), and c retinal endothelial cells 96 h after infection with the ZIKV. Immunofluorescence staining of ZIKV-infected endothelial cells after 96 h with the Flavivirus 4G2 antibody. d All images were taken on a Nikon TE2000S microscope mounted with a charge-coupled device (CCD) camera at ×200 magnification. For fluorescent images, 4′,6-diamidino-2-phenylindole (DAPI) was used to stain the nuclei blue