Fig. 3

Overexpression of miR-142a-5p affects the differentiation of CD4⁺ T cells. Naïve CD4⁺ T cells were isolated from mouse splenocytes to a purity of approximately 95% (a). miR-142a-3p, miR-142a-5p, and negative control sequences were transfected into CD4⁺ T cells which were activated and polarized under relevant cytokine regimens. The frequencies of Th1, Th17, and Treg cells in CD4⁺ T cells were determined by intracellular staining and flow cytometry after 4 days. Representative dot plots and the percentages of IFN-γ, IL-17, and FoxP3 immunopositive cells within the CD4⁺ T cells (b). Average cell frequencies (c). Data are shown as mean ± SEM, n = 3. Data are from a single experiment representative of three independent experiments.(*p < 0.05, one-way ANOVA)