Fig. 5
Clozapine was protective independent of regulatory T cells. a–f Tregs were neutralized in mice 3 days before EAE induction and maintained throughout the experiment. Clozapine was added to drinking water 1 day before induction for EAE and mice scored daily for disease (0: normal to 5: moribund). Disease score of mice (a). Disease burden assessed by area under the curve analysis (b). Onset of clinical disease (c). Peak disease score reached of mice with disease (d). Shown are the means and SEM of individual mice from two experiments combined (a–d n = 5 mice/group). *p < 0.05, **p < 0.01 by two-way ANOVA and Sidak’s multiple comparison test. ••p < 0.01 by Mann-Whitney test. e–f IFN-γ (e) and IL-17A (f) in the supernatant after MOG35-55-peptide-specific recall for 72 h. Shown are means and SEM of individual mice from one experiment (n = 5 mice in each group)