Fig. 3

GFAP and AQP4 distribution in the corpus callosum (CC). a(A–F) Representative images of double immunofluorescent staining for GFAP and AQP4 in the corpus callosum (CC). a(A) Sham-std; a(B) Sham-3 h; a(C) Sham-full; a(D) BCAS-std; a(E) BCAS-3 h; a(F) BCAS-full. Scale bar represents 10 μm. a(A-C) In Sham subgroups, AQP4 was normally distributed within the astrocytic end-feet around the vessels (arrow heads). a(D) and a(F) In BCAS subgroups, AQP4 was abnormally aggregated at the periphery of GFAP-positive astrocytes/clasmatodendrocytes, especially in BCAS-std and BCAS-full subgroups (arrows). a(E) In limited exposure to EE (BCAS-3 h), less abnormal distribution of AQP4 was seen in the CC. b Histogram showing % AQP4 dislocation in each group. EE, especially by limited exposure to EE (BCAS-3 h) attenuated AQP4 dislocation in the CC (**P < 0.01). Sham-std, n = 11; Sham-3 h, n = 11; Sham-full, n = 11; BCAS-std, n = 13, BCAS-3 h, n = 10; BCAS-full, n = 12