Microglia activation is essential for BMP7-mediated retinal reactive gliosis

Table 1 The primers used for qPCR analysis

Gene	Primer	Sequence	Product length
Gm-Csf	Forward	AGTCGTCTCTAACGAGTTCTCC	178
Gm-Csf	Reverse	AACTTGTGTTTCACAGTCCGTT	178
Csf1	Forward	ACCAAGAACTGCAACAACAGC	91
Csf1	Reverse	GGGTGGCTTTAGGGTACAGG	91
Inf-α	Forward	CAAGCCATCCCTGTCCTGAG	131
Inf-α	Reverse	TCATTGAGCTGCTGGTGGAG	131
Inf-γ	Forward	CAACAGCAAGGCGAAAAAGGA	90
Inf-γ	Reverse	AGCTCATTGAATGCTTGGCG	90
Il-1β	Forward	TGTCTGAAGCAGCTATGGCAA	141
Il-1β	Reverse	GACAGCCCAGGTCAAAGGTT	141
Il6	Forward	ACTTCACAAGTCGGAGGCTT	111
Il6	Reverse	TGCAAGTGCATCATCGTTGT	111
VEGF	Forward	ACTGGACCCTGGCTTTACTG	74
VEGF	Reverse	CTCTCCTTCTGTCGTGGGTG	74
Tnf-α	Forward	TAGCCCACGTCGTAGCAAAC	136
Tnf-α	Reverse	ACAAGGTACAACCCATCGGC	136
Ccl5	Forward	TGCCCACGTCAAGGAGTATTT	111
Ccl5	Reverse	ACCCACTTCTTCTCTGGGTTG	111
Thbs1	Forward	GCCACAGTTCCTGATGGTGA	149
Thbs1	Reverse	TTGAGGCTGTCACAGGAACG	149
Thbs2	Forward	GGGAGGACTCAGACCTGGAT	105
Thbs2	Reverse	CGGAATTTGGCAGTTTGGGG	105
Cd45	Forward	TGACCATGGGTTTGTGGCTC	134
Cd45	Reverse	TTGAGGCAGAAGAAGGGCAT	134
Cd68	Forward	AAGGGGGCTCTTGGGAACTA	139
Cd68	Reverse	AAGCCCTCTTTAAGCCCCAC	139
Iba1	Forward	ACGAACCCTCTGATGTGGTC	118
Iba1	Reverse	TGAGGAGGACTGGCTGACTT	118
Irf8	Forward	CGGATATGCCGCCTATGACA	73
Irf8	Reverse	CTTGCCCCCGTAGTAGAAGC	73
Sdha	Forward	GGACAGGCCACTCACTCTTAC	130
Sdha	Reverse	CACAGTGCAATGACACCACG	130
Srp14	Forward	CCTCGAGCCCGCAGAAAA	134
Srp14	Reverse	CGTCCATGTTGGCTCTCAGT	134

ISSN: 1742-2094