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Table 2 Effects of acute binge ethanol and/or microglial depletion on brain gene expression

From: Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal

 

CON

–M

E

–M + E

Microglial markers

 Iba1a, b, c

100 ± 1A

6 ± 0.4B

68 ± 3C

6 ± 1B

 CD11ba, b, c

100 ± 2A

3 ± 1B

57 ± 2C

5 ± 1B

 CD45a

100 ± 7

52 ± 7

121 ± 15

69 ± 5

 MHCII

100 ± 8

68 ± 22

67 ± 4

48 ± 15

M1 microglial markers

 CD68a, b

100 ± 6

17 ± 2

136 ± 9

42 ± 4

 CD86a, b, c

100 ± 1A

2 ± 0.3B

127 ± 6C

5 ± 2B

 iNOSa, b, c

100 ± 24A

135 ± 37A

170 ± 37A

812 ± 145B

 NOX2a

100 ± 13

46 ± 11

137 ± 14

51 ± 10

M2 microglial markers

 CD206a

100 ± 2

4 ± 0.2

91 ± 5

5 ± 0.3

 CD163a, b

100 ± 7

15 ± 3

127 ± 11

30 ± 3

 Arg1a, b

100 ± 8

103 ± 10

108 ± 13

157 ± 11

 Ym1

100 ± 15

133 ± 32

194 ± 36

197 ± 57

Pro-inflammatory cytokines

 IL-1βb

100 ± 6

95 ± 14

143 ± 17

174 ± 16

 TNFαa, b, c

100 ± 8A

68 ± 11A

600 ± 90B

104 ± 26A

 IL-6b

100 ± 9

92 ± 6

131 ± 13

164 ± 9

 Ccl2b

100 ± 12

127 ± 36

1002 ± 308

355 ± 60

Anti-inflammatory cytokines

 IL-10b, c

100 ± 5A

91 ± 7A

112 ± 11A

152 ± 9B

 IL-4b, c

100 ± 7A, B

84 ± 5A

186 ± 10B

291 ± 40C

 IL-1raa, b

100 ± 5

113 ± 19

117 ± 17

173 ± 16

 TGF-β1a

100 ± 3

44 ± 3

107 ± 11

58 ± 8

CNS cell types

 NeuNa

100 ± 4

120 ± 5

108 ± 5

125 ± 10

 MAP2

100 ± 3

106 ± 4

108 ± 4

102 ± 5

 DCXb

100 ± 7

97 ± 7

84 ± 3

83 ± 6

 GFAPa

100 ± 5

148 ± 11

111 ± 11

145 ± 10

 S100β

100 ± 5

91 ± 5

91 ± 4

95 ± 6

 CNP

100 ± 3

93 ± 7

86 ± 2

92 ± 7

 MBP

100 ± 9

91 ± 6

84 ± 7

85 ± 5

 ICAM2b, c

100 ± 6A

83 ± 5A

108 ± 11A

144 ± 8B

 Pecam1

100 ± 3

97 ± 4

103 ± 9

110 ± 7

Toll-like receptors

 TLR2a, b

100 ± 4

64 ± 7

133 ± 8

94 ± 7

 TLR3

100 ± 8

103 ± 6

99 ± 3

100 ± 5

 TLR4b

100 ± 8

82 ± 9

137 ± 11

125 ± 11

 TLR7a, b, c

100 ± 5A

24 ± 4B

152 ± 6C

38 ± 3B

Neuron-microglia signaling

 CX3CL1a, b, c

100 ± 3A

93 ± 2A

96 ± 4A

75 ± 3B

 CX3CR1a

100 ± 5

1 ± 0.1

112 ± 15

4 ± 1

 CD200

100 ± 5

106 ± 5

102 ± 3

104 ± 3

 CD200R1

100 ± 16

70 ± 9

105 ± 16

91 ± 8

 CSF1

100 ± 2

120 ± 5

113 ± 5

104 ± 5

 CSF1Ra

100 ± 5

2 ± 0.3

91 ± 4

4 ± 1

Neurotrophins

 BDNFa, c

100 ± 4A, B

101 ± 4A, B

113 ± 6A

86 ± 5B

 NGF

100 ± 6

103 ± 3

96 ± 4

107 ± 4

 NT3

100 ± 7

111 ± 9

96 ± 6

117 ± 10

 NT4/5a, b

100 ± 6

115 ± 15

131 ± 10

176 ± 17

Cytokine receptors

 IL-1R1a, b

100 ± 4

113 ± 8

130 ± 6

189 ± 20

 TNFR1b

100 ± 2

101 ± 5

131 ± 4

120 ± 6

 TNFR2a, b

100 ± 5

55 ± 2

124 ± 5

70 ± 6

 CCR2

100 ± 11

110 ± 21

131 ± 22

144 ± 13

Immediate-early genes

 c-Fos

100 ± 6

99 ± 13

155 ± 26

123 ± 20

 EGR1

100 ± 9

96 ± 4

121 ± 8

92 ± 13

 Arc

100 ± 10

81 ± 13

105 ± 17

75 ± 19

 c-Junb, c

100 ± 7A, B

81 ± 5A

105 ± 5A, B

116 ± 8B

Apoptosis pathways

 FasLa, b, c

100 ± 6A

98 ± 11A

121 ± 8A

186 ± 20B

 FasRb

100 ± 3

107 ± 7

146 ± 17

210 ± 25

 TL1Ab

100 ± 9

88 ± 8

127 ± 25

157 ± 12

 DR3a

100 ± 5

71 ± 7

91 ± 9

55 ± 9

 TRAILb

100 ± 5

108 ± 5

88 ± 7

67 ± 13

 DR5a

100 ± 6

106 ± 5

91 ± 6

139 ± 17

 LTαa, b, c

100 ± 5A

95 ± 10A

120 ± 19A

233 ± 25B

 FADD

100 ± 3

98 ± 5

99 ± 4

114 ± 14

 Caspase-3

100 ± 4

112 ± 4

112 ± 4

130 ± 12

Clock genes

 Per1b

100 ± 3

111 ± 8

155 ± 6

185 ± 41

 Per2b, c

100 ± 8A

123 ± 5A, B

202 ± 7C

147 ± 12B

 BMAL1

100 ± 5

95 ± 5

104 ± 4

110 ± 6

Neurotransmitter systems

 CRFa

100 ± 8

87 ± 7

93 ± 6

73 ± 7

 DAT

100 ± 5

98 ± 7

92 ± 4

100 ± 4

 GluR1b

100 ± 2

101 ± 4

123 ± 3

121 ± 10

 NPY

100 ± 2

95 ± 4

96 ± 4

96 ± 3

Other

 Glucocorticoid Receptor

100 ± 5

99 ± 3

96 ± 3

90 ± 6

 HMGB1

100 ± 4

103 ± 7

101 ± 4

109 ± 4

 COX2b

100 ± 7

106 ± 5

140 ± 7

151 ± 9

 MMP9b

100 ± 2

106 ± 7

125 ± 6

133 ± 8

 IL-12

100 ± 5

112 ± 7

99 ± 8

101 ± 6

 IL-34

100 ± 7

101 ± 6

96 ± 7

262 ± 80

 NLRP3a, b

100 ± 3

27 ± 4

159 ± 22

57 ± 6

 Nogo

100 ± 4

114 ± 6

124 ± 5

116 ± 3

  1. Values are for mRNA levels set to 100% of controls. Control or microglia-depleted mice were gavaged with either water or ethanol (6 g/kg, 25% v/v) and sacrificed 18 h post-treatment
  2. A, B, C, D = means with different letters are significantly different (p < 0.05, Tukey’s post hoc test) from each other. Means with the same letters are not significantly different from each other. Only means with significant interactions are labeled with letters. Data represent mean ± SEM
  3. CON column represents water-treated control mice, —M, microglia-depleted mice, E, ethanol-treated mice, —M + E, microglia-depleted and ethanol-treated mice
  4. aMain effect of microglial depletion
  5. bMain effect of ethanol treatment
  6. cInteraction