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Table 2 Effects of acute binge ethanol and/or microglial depletion on brain gene expression

From: Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal

  CON –M E –M + E
Microglial markers
 Iba1a, b, c 100 ± 1A 6 ± 0.4B 68 ± 3C 6 ± 1B
 CD11ba, b, c 100 ± 2A 3 ± 1B 57 ± 2C 5 ± 1B
 CD45a 100 ± 7 52 ± 7 121 ± 15 69 ± 5
 MHCII 100 ± 8 68 ± 22 67 ± 4 48 ± 15
M1 microglial markers
 CD68a, b 100 ± 6 17 ± 2 136 ± 9 42 ± 4
 CD86a, b, c 100 ± 1A 2 ± 0.3B 127 ± 6C 5 ± 2B
 iNOSa, b, c 100 ± 24A 135 ± 37A 170 ± 37A 812 ± 145B
 NOX2a 100 ± 13 46 ± 11 137 ± 14 51 ± 10
M2 microglial markers
 CD206a 100 ± 2 4 ± 0.2 91 ± 5 5 ± 0.3
 CD163a, b 100 ± 7 15 ± 3 127 ± 11 30 ± 3
 Arg1a, b 100 ± 8 103 ± 10 108 ± 13 157 ± 11
 Ym1 100 ± 15 133 ± 32 194 ± 36 197 ± 57
Pro-inflammatory cytokines
 IL-1βb 100 ± 6 95 ± 14 143 ± 17 174 ± 16
 TNFαa, b, c 100 ± 8A 68 ± 11A 600 ± 90B 104 ± 26A
 IL-6b 100 ± 9 92 ± 6 131 ± 13 164 ± 9
 Ccl2b 100 ± 12 127 ± 36 1002 ± 308 355 ± 60
Anti-inflammatory cytokines
 IL-10b, c 100 ± 5A 91 ± 7A 112 ± 11A 152 ± 9B
 IL-4b, c 100 ± 7A, B 84 ± 5A 186 ± 10B 291 ± 40C
 IL-1raa, b 100 ± 5 113 ± 19 117 ± 17 173 ± 16
 TGF-β1a 100 ± 3 44 ± 3 107 ± 11 58 ± 8
CNS cell types
 NeuNa 100 ± 4 120 ± 5 108 ± 5 125 ± 10
 MAP2 100 ± 3 106 ± 4 108 ± 4 102 ± 5
 DCXb 100 ± 7 97 ± 7 84 ± 3 83 ± 6
 GFAPa 100 ± 5 148 ± 11 111 ± 11 145 ± 10
 S100β 100 ± 5 91 ± 5 91 ± 4 95 ± 6
 CNP 100 ± 3 93 ± 7 86 ± 2 92 ± 7
 MBP 100 ± 9 91 ± 6 84 ± 7 85 ± 5
 ICAM2b, c 100 ± 6A 83 ± 5A 108 ± 11A 144 ± 8B
 Pecam1 100 ± 3 97 ± 4 103 ± 9 110 ± 7
Toll-like receptors
 TLR2a, b 100 ± 4 64 ± 7 133 ± 8 94 ± 7
 TLR3 100 ± 8 103 ± 6 99 ± 3 100 ± 5
 TLR4b 100 ± 8 82 ± 9 137 ± 11 125 ± 11
 TLR7a, b, c 100 ± 5A 24 ± 4B 152 ± 6C 38 ± 3B
Neuron-microglia signaling
 CX3CL1a, b, c 100 ± 3A 93 ± 2A 96 ± 4A 75 ± 3B
 CX3CR1a 100 ± 5 1 ± 0.1 112 ± 15 4 ± 1
 CD200 100 ± 5 106 ± 5 102 ± 3 104 ± 3
 CD200R1 100 ± 16 70 ± 9 105 ± 16 91 ± 8
 CSF1 100 ± 2 120 ± 5 113 ± 5 104 ± 5
 CSF1Ra 100 ± 5 2 ± 0.3 91 ± 4 4 ± 1
Neurotrophins
 BDNFa, c 100 ± 4A, B 101 ± 4A, B 113 ± 6A 86 ± 5B
 NGF 100 ± 6 103 ± 3 96 ± 4 107 ± 4
 NT3 100 ± 7 111 ± 9 96 ± 6 117 ± 10
 NT4/5a, b 100 ± 6 115 ± 15 131 ± 10 176 ± 17
Cytokine receptors
 IL-1R1a, b 100 ± 4 113 ± 8 130 ± 6 189 ± 20
 TNFR1b 100 ± 2 101 ± 5 131 ± 4 120 ± 6
 TNFR2a, b 100 ± 5 55 ± 2 124 ± 5 70 ± 6
 CCR2 100 ± 11 110 ± 21 131 ± 22 144 ± 13
Immediate-early genes
 c-Fos 100 ± 6 99 ± 13 155 ± 26 123 ± 20
 EGR1 100 ± 9 96 ± 4 121 ± 8 92 ± 13
 Arc 100 ± 10 81 ± 13 105 ± 17 75 ± 19
 c-Junb, c 100 ± 7A, B 81 ± 5A 105 ± 5A, B 116 ± 8B
Apoptosis pathways
 FasLa, b, c 100 ± 6A 98 ± 11A 121 ± 8A 186 ± 20B
 FasRb 100 ± 3 107 ± 7 146 ± 17 210 ± 25
 TL1Ab 100 ± 9 88 ± 8 127 ± 25 157 ± 12
 DR3a 100 ± 5 71 ± 7 91 ± 9 55 ± 9
 TRAILb 100 ± 5 108 ± 5 88 ± 7 67 ± 13
 DR5a 100 ± 6 106 ± 5 91 ± 6 139 ± 17
 LTαa, b, c 100 ± 5A 95 ± 10A 120 ± 19A 233 ± 25B
 FADD 100 ± 3 98 ± 5 99 ± 4 114 ± 14
 Caspase-3 100 ± 4 112 ± 4 112 ± 4 130 ± 12
Clock genes
 Per1b 100 ± 3 111 ± 8 155 ± 6 185 ± 41
 Per2b, c 100 ± 8A 123 ± 5A, B 202 ± 7C 147 ± 12B
 BMAL1 100 ± 5 95 ± 5 104 ± 4 110 ± 6
Neurotransmitter systems
 CRFa 100 ± 8 87 ± 7 93 ± 6 73 ± 7
 DAT 100 ± 5 98 ± 7 92 ± 4 100 ± 4
 GluR1b 100 ± 2 101 ± 4 123 ± 3 121 ± 10
 NPY 100 ± 2 95 ± 4 96 ± 4 96 ± 3
Other
 Glucocorticoid Receptor 100 ± 5 99 ± 3 96 ± 3 90 ± 6
 HMGB1 100 ± 4 103 ± 7 101 ± 4 109 ± 4
 COX2b 100 ± 7 106 ± 5 140 ± 7 151 ± 9
 MMP9b 100 ± 2 106 ± 7 125 ± 6 133 ± 8
 IL-12 100 ± 5 112 ± 7 99 ± 8 101 ± 6
 IL-34 100 ± 7 101 ± 6 96 ± 7 262 ± 80
 NLRP3a, b 100 ± 3 27 ± 4 159 ± 22 57 ± 6
 Nogo 100 ± 4 114 ± 6 124 ± 5 116 ± 3
  1. Values are for mRNA levels set to 100% of controls. Control or microglia-depleted mice were gavaged with either water or ethanol (6 g/kg, 25% v/v) and sacrificed 18 h post-treatment
  2. A, B, C, D = means with different letters are significantly different (p < 0.05, Tukey’s post hoc test) from each other. Means with the same letters are not significantly different from each other. Only means with significant interactions are labeled with letters. Data represent mean ± SEM
  3. CON column represents water-treated control mice, —M, microglia-depleted mice, E, ethanol-treated mice, —M + E, microglia-depleted and ethanol-treated mice
  4. aMain effect of microglial depletion
  5. bMain effect of ethanol treatment
  6. cInteraction