Table 1 CUS procedure for rats
Week | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | Sunday |
|---|---|---|---|---|---|---|---|
1 | Hot environment 40 °C, 5 min | Water deprivation 24 h | Swimming in cold water 5 min | Cage shake 30 min | Light-dark cycle reversal | Hot environment 40 °C, 5 min | Food deprivation |
2 | Swimming in cold water 5 min | Light-dark cycle reversal | Hot environment 40 °C, 5 min | Water deprivation 24 h | Cage shake 30 min | Cage tilt 2 h Food deprivation 24 h | Swimming in cold water 5 min |
3 | Cage shake 30 min | Hot environment 40 °C, 5 min | Food deprivation 24 h | Light-dark cycle reversal | Water deprivation 24 h | Swimming in cold water 5 min | Hot environment 40 °C, 5 min |