Table 2 CUS procedure for mice
Week | Monday | Tuesday | Wednesday | Thursday | Friday | Saturday | Sunday |
|---|---|---|---|---|---|---|---|
1 | Restraint 2 h Hot environment (40 °C, 5 min) | Cage tilt 2 h Food and water deprivation 24 h | Swimming in cold water 5 min | Cage shake 30 min Light inversion 12 h | Wet bedding 6 h Stroboscopic light 12 h | Cage tilt 2 h Hot environment (40 °C, 5 min) | Cage shake 30 min Food and water deprivation 24 h |
2 | Cage tilt 2 h | Swimming in cold water 5 min Light inversion 12 h | Restraint 2 h Stroboscopic light 12 h | Hot environment (40 °C, 5 min) Wet bedding 6 h | Restraint 2 h Hot environment (40 °C, 5 min) | Cage tilt 2 h Food and water deprivation 24 h | Swimming in cold water 5 min |
3 | Cage shake 30 min Light inversion 12 h | Wet bedding 6 h Stroboscopic light 12 h | Swimming in cold water 5 min hot environment (40 °C, 5 min) | Cage shake 30 min Food and water deprivation 24 h | Cage tilt 2 h | Swimming in cold water 5 min Light inversion 12 h | Restraint 2 h Stroboscopic light 12 h |
4 | Restraint 2 h Wet bedding 6 h | Restraint 2 h Hot environment (40 °C, 5 min) | Cage tilt 2 h Food and water deprivation 24 h | Swimming in cold water 5 min | Cage shake 30 min Light inversion 12 h | Wet bedding 6 h Stroboscopic light 12 h | Cage tilt 2 h Hot environment (40 °C, 5 min) |
5 | Cage shake 30 min Food and water deprivation 24 h | Swimming in cold water 5 min | Wet bedding 6 h Light inversion 12 h | Restaint 2 h Stroboscopic light 12 h | Hot environment (40 °C, 5 min) Wet bedding 6 h | Restraint 2 h Cage shake 30 min | Cage tilt 2 h Hot environment (40 °C, 5 min) |