Table 2 Gene primer sequences for RT-rt-PCR analysis
Gene | Primer sequences (5′-3′) | Expected size (bp) | Gene bank accession number |
|---|---|---|---|
PAFR | F: CAACGAGGGCGACTGGATT | 97 | D50872.1 [93] |
R: GACACCCAAAAAGGCCACACT | |||
PAF-AH I α1 | F: ACACAGCATGTACTCTGGCG | 293 | BC067015.1 [93] |
R: GCATCTAAGAAGTGGGCTCG | |||
PAF-AH I α2 | F: AGAATGCCAAGGTGAACCAG | 127 | BC056211.2 [94] |
R: AAATCAAACATGTCGTGCCA | |||
PAF-AH I LIS1 | F: GATGACAAGACCCTCCGTGT | 240 | NM_013625.4 [95] |
R: GAGCTCAAATGGGGTAACCA | |||
PAF-AH II | F: ATCAAGGAAGGGGAGAAGGA | 200 | BC021890.1 [96] |
R: AAGGAGTGACCCATCACGGC | |||
TNF-α | F: CATCTTCTCAAAATTCGAGTGACAA | 175 | D84199.2 [97] |
R: TGGGAGTAGACAAGGTACAACCC | |||
IL-1β | F: GAAAGACGGCACACCCACC | 83 | BC011437.1 [98] |
R: AGACAAACCGCTTTTCCATCTTC | |||
IL-6 | F: TGGAGTCACAGAAGGAGTGGCTAAG | 155 | BC132458.1 |
R: TCTGACCACAGTGAGGAATGTCCAG | |||
INF-γ | F: TCAAGTGGCATAGATGTGGAAGAA | 92 | BC119063.1 [97] |
R: TGGCTCTGCAGGATTTTCATG | |||
iNOS | F: CAGCTGGGCTGTACAAACCTT | 95 | BC062378.1 [97] |
R: CATTGGAAGTGAAGCGTTTCG | |||
PPAR-α | F: CCCTGAACATCGAGTGTCGAA | 142 | BC016892.1 [99] |
R: TTGCAGCTCCGATCACACTT | |||
PPAR- γ | F: TGTCGGTTTCAGAAGTGCCTT | 146 | AB644275.1 |
R: GCTCGCAGATCAGCAGACTCT | |||
YM1 | F: ACCCCTGCCTGTGTACTCACCT | 183 | BC061154.1 [100] |
R: CACTGAACGGGGCAGGTCCAAA | |||
CD206 | F: TCTTTGCCTTTCCCAGTCTCC | 241 | NM_008625.2 [101] |
R: TGACACCCAGCGGAATTTC | |||
CD16 | F: TTTGGACACCCAGATGTTTCAG | 163 | NM_010188.5 [101] |
R: GTCTTCCTTGAGCACCTGGATC | |||
CD32 | F: AATCCTGCCGTTCCTACTGATC | 187 | BC038070.1 [101] |
R: GTGTCACCGTGTCTTCCTTGAG | |||
CD86 | F: TTGTGTGTGTTCTGGAAACGGAG | 202 | BC013807.1 [101] |
R: AACTTAGAGGCTGTGTTGCTGGG | |||
COX-2 | F: CCACTTCAAGGGAGTCTGGA | 197 | NM_011198.3 [102] |
R: AGTCATCTGCTACGGGAGGA | |||
GAPDH | F: GCCAAGGCTGTGGGCAAGGT | 112 | GU214026.1 [103] |
R: TCTCCAGGCGGCACGTCAGA |