Fig. 1

HSP70 expression was higher in the ipsilateral frontal cortex (a), hippocampus (b), striatum (c), and hypothalamus (d) in exercise-preconditioned (EP⁺) rats with traumatic brain injury (TBI) or without TBI (sham). A: EP⁻ rats were given a sham operation and intracerebral administration of siRNA-vector (EP⁻ + sham + siRNA-vector). B: EP⁻ rats were given a sham operation and intracerebral siRNA-HSP70 (EP⁻ + sham + siRNA-HSP70). C: EP⁺ + sham rats were given siRNA-vector (EP⁺ + Sham + siRNA-vector). D: EP⁺ + sham rats were given intracerebral siRNA-HSP70 (EP⁺ + sham + siRNA-HSP70). A’: EP⁻ + TBI⁺ rats were given intracerebral siRNA-vector (EP⁻ + TBI + siRNA-vector). B’: EP⁻ + TBI⁺ rats were given intracerebral siRNA-HSP70 (EP⁻ + TBI + siRNA-HSP70). C’: EP⁺ + TBI⁺ rats were given intracerebral siRNA-vector (EP⁺ + TBI + siRNA-vector). D’: EP⁺ + TBI⁺ rats were given intracerebral siRNA-HSP70 (EP⁺ + TBI + siRNA-HSP70). Cerebral expression of HSP70 was assessed by Western blotting 3 days after a TBI or a sham operation. The gels presented are representatives of results from three separate experiments. Densitometry readings of gel bands expressed as arbitrary units of relative intensities to that of EP⁻ + TBI⁻ + siRNA-vector control. Values represent mean ± SD of three separate experiments. *P < 0.05 for C or C' vs. A; #P < 0.05 for D vs. C; †P < 0.05 for C’ vs. A’; ‡P < 0.05 for C’ vs. B’; and §P < 0.05 for D’ vs. C’