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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Exercise attenuates neurological deficits by stimulating a critical HSP70/NF-κB/IL-6/synapsin I axis in traumatic brain injury rats

Fig. 8

HSP70-mediated exercise preconditioning (EP+) induces increased expression of NF-κB in the ipsilateral frontal cortex was higher than that in HSP70-mediated EP+ rats. ChIP analysis was used to evaluate the extent of NF-κB binding to the DNA elements in the IL-6 promoter site 1 (a) and site 2 (b) regions using “EpiTect ChIP qPCR Assays kit” from Qiagen in (EP− + sham + siRNA-vector) rats, (EP+ + sham + siRNA-vector) rats, (EP+ + sham + siRNA-HSP70) rats, (EP− + TBI + siRNA-vector) rats, (EP+ + TBI + siRNA-vector) rats, and (EP+ + TBI+ + siRNA-HSP70) rats. The increased levels of HSP70 in front cortex caused by EP+ were significantly attenuated by depleting HSP70 levels with shHSP70 preconditioning. *P < 0.05 for EP+ + siRNA-vector vs. EP− + siRNA-vector and † P < 0.05 for EP+ + siRNA-vector vs. EP+ + siRNA-HSP70

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Journal of Neuroinflammation

ISSN: 1742-2094