Fig. 4

Expression and activation of GPR120 and GPR40. The expression of GPR120 (a and c) and GPR40 (b and d) transcripts were evaluated by real-time PCR in samples collected from the small intestine, liver, white adipose tissue (WAT), frontal cortex, occipital cortex, hippocampus, and hypothalamus (a and b) of lean Swiss mice or in samples prepared from the mHypoA 2/29 CLU189 and BV2 cell lines (c and d). In e–g, lean Swiss mice were subjected to intracerebroventricular cannulation and treated with a single dose of GW9508 (2.0 μl, 1.0 mM); samples were collected after 90 min for the evaluation of GPR120 (E) or GPR40 (g) transcript expression by real-time PCR. In addition, protein samples were utilized in immunoprecipitation experiments using an anti-β-arrestin-2 antibody; immunoprecipitates were separated by SDS-PAGE, transferred to nitrocellulose membranes, and immunoblotted (IB) with GPR120 or β-arrestin-2 antibodies (f). In all experiments, n = 5. In C and D, *p < 0.05 vs. CLU189; in E and F, *p < 0.05 vs. vehicle