Fig. 4

PLX does not alter cerebellar astrogliosis in SCA1 mice. a Representative confocal images of 3-month-old mice stained with an astrocyte-specific anti-GFAP antibody on the WT and ATXN1[82Q] mice fed regular (control) or PLX chow (PLX). Scale bar, 50 μm. b Quantification of GFAP intensity. c qRT-PCR was used to determine expression of astrocyte genes in cerebellar samples from PLX and control SCA1 and their wild-type littermate controls with reference to control WT mice (represented by green line) and normalized to 18S RNA. N ≥ 3. Each dot represents one mouse, and values indicate mean ± SEM. Asterisk indicates p < 0.05 by one-way ANOVA followed by Bonferroni post hoc test, presence of n.s. indicates that p > 0.05 and that data was not significantly different