Receptor tyrosine kinases play a significant role in human oligodendrocyte inflammation and cell death associated with the Lyme disease bacterium Borrelia burgdorferi

Table 4 Average fold change^a in chemokine/cytokine levels with EGFR, FGFR, PDGFR, and src family kinase inhibitors

Chemokine-cytokine/treatment	CCL2	CXCL8	CCL5	CXCL(1,2,3)	IL-6
Bb + 10 μM EGFR inhibitor	1.466^b (±0.330)	0.515 (±0.068)	0.674 (±0.118)	0.657^b (±0.133)	0.836^b (±0.296)
Bb + 100 nM PDGFRα/β inhibitor	1.733 (±0.461	0.797 (±0.174)	0.977 (±0.123)	0.738 (±0.124)	0.478 (±0.048)
Bb + 5 μM FGFR inhibitor	0.920 (±0.125)	0.504 (±0.019)	0.911 (±0.077)	0.425 (±0.028)	0.461 (±0.047)
Bb + 1 μM FGFR inhibitor	0.753 (±0.165)	0.670 (±0.047)	0.573 (±0.081)	0.652 (±0.043)	0.706 (±0.082)
Bb + 500 nM FGFR inhibitor	0.756 (±0.054)	0.693 (±0.028)	0.760 (±0.190)	0.697 (±0.041)	0.634 (±0.027)
Bb + 5 μM src family inhibitor	1.184 (±0.168)	1.258 (±0.079)	1.051 (±0.064)	0.964 (±0.022)	1.465 (±0.080)
Bb + 1 μM src family inhibitor	0.901 (±0.173)	0.993^b (±0.081)	0.778 (±0.125)	1.109 (±0.091)	1.035 (±0.082)
Bb + 500 nM src family inhibitor	0.893 (±0.122)	0.919 (±0.079)	0.847 (±0.036)	1.061 (±0.100)	1.044 (±0.137)

^aFold change was calculated as B. burgdorferi (MOI 10:1) + solvent control/Bn burgdorferi + inhibitor for each experiment at 48 h post exposure. Average fold change values were calculated from the mean of two to three independent experiments, with standard error of the mean within parenthesis. Fold change values >1 indicate a downregulation of chemokine/cytokine levels, and values <1 indicate an increase in those levels. Values in italics or those in bold indicate statistically significant upregulation or downregulation respectively, in a majority (2/2 or 2/3) of experiments
^bInconclusive results

ISSN: 1742-2094