Fig. 3

miR-181c suppresses MLL1 expression via 3′-UTR binding. a Human MLL1 3′-UTR fragments containing either wild-type or mutated miR-181c-binding sites cloned downstream of the luciferase reporter gene. Mutations were generated in MLL1’s 3′-UTR sequences complementary to miR-181c’s seed region. b, c Luciferase activity assays using reporters with either wild-type or mutant human MLL1’s 3′-UTRs performed following co-transfection with miR-181c mimics or NC-miR in b microglia or c HEK293T cells. Luciferase activity of the NC-miR transfection (which was set to unity) used for normalization. d Microglia transfected with miR-181c mimic or NC-miR duplexes. After 48 h, MLL1’s mRNA and protein expression were evaluated by qRT-PCR and Western blotting, respectively. Control indicates untreated control microglia. NC-miR indicates the negative control for the miR-181c mimics. All results presented as means ± SEMs from three independent experiments. *p < 0.05 versus same-condition control, †p < 0.05 versus same-condition NC-miR