Fig. 4

Assessment of NF-κB activity by luciferase assay. a Relative NF-κB luciferase activity in microglia and HEK293T cells under the various experimental conditions. b MLL1 knockdown in MLL1-silenced microglia was validated by qRT-PCR and Western blotting. c Relative NF-κB luciferase activity in MLL1+ and MLL1-silenced microglia under the various experimental conditions. Control condition indicates untreated cells, while thrombin indicates thrombin-treated cells. NC-miR indicates the negative control for the miR-181c mimics. All results presented as means ± SEMs from three independent experiments. *p < 0.05 versus same-condition control, †p < 0.05 versus same-condition NC-miR, ‡p < 0.05, same-condition wild-type versus MLL1-siRNA microglia