Fig. 7

MCP1+ cells are mainly microglia and some CCR2+ cells are infiltrating monocytes in the motor cortex. a, b Representative images of immunohistochemistry for neuronal marker NeuN, MCP1+ cells (a, a’, a”), and CCR2+ cells (b, b’, b”) reveal that neither MCP1+ nor CCR2+ cells are mature neurons. c, e Representative images of immunohistochemistry for monocyte/macrophage lineage marker CD11b (c, c’, c”), microglia marker Iba1 (e, e’, e”), and MCP1+ cells demonstrate co-localization and microglial lineage. d, f Representative images of immunohistochemistry for microglia/monocyte lineage marker CD11b (d, d’, d”), microglia marker Iba1 (f, f’, f”), and CCR2+ cells show close cellular interaction but lack of co-localization with microglia markers. g, i Representative images of immunohistochemistry for astrocyte (GFAP+: g, g’, g”) and infiltrating monocyte (Ly6C+: i, i’, i”) markers reveal lack of co-localization with MCP1+ cells. h, j Representative images of immunohistochemistry for astrocyte (GFAP+: h, h’, h”) and infiltrating monocyte (Ly6C+: j, j’, j”) markers reveal that CCR2+ cells are in close contact with astrocytes (GFAP+) but do not express GFAP themselves. However, a subpopulation of CCR2+ cells co-localize with infiltrating monocyte marker (Ly6C). Arrows point to the cells of interest. Scale bar = 20 μm