Fig. 6

Resident microglia numbers and MHC class II expression on individual microglial cells increase within the OB during latency. a Representative flow plot showing CD45^int, CD11b⁺ cells from OB defined as microglia (MG). b Total number of MG residing in the OB at times post infection compared to uninfected (UI) OB, *p < 0.05 between 7 and 60 DPI by a Dunn’s post comparisons analysis. c Representative histogram demonstrating an increase in MG mean fluorescence intensity (MFI) of major histocompatibility complex class II (MHC II) expression comparing 60 DPI (black) to UI (grey) OB MG. d Total MG MFI of MHC II expression as determined by flow cytometry, p < *0.05 comparing 60 DPI to UI controls with a Dunn’s post-test. For b and d UI n = 4, 7 DPI, n = 5, and 30 and 60 DPI, n = 7